Coding

Part:BBa_K4477001:Design

Designed by: Miranda Khoury   Group: iGEM22_Virginia   (2022-09-06)
Revision as of 07:24, 12 October 2022 by Mirandakhoury (Talk | contribs) (→‎Design Notes)


Human/mouse anti-apoB(MDA) (anti-oxLDL) IgG antibody - light chain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 382


Design Notes

Amino acid sequence acquired from database was reverse transcribed and codon optimized for expression in E. coli B (the parent strain of SHuffle). Illegal restriction sites were removed via codon optimization.

Source

Anti-apoB(MDA) light chain amino acid sequence acquired from IMGT: https://www.imgt.org/3Dstructure-DB/cgi/details.cgi?pdbcode=9635

References