Coding

Part:BBa_K4229062

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-30)
Revision as of 05:53, 12 October 2022 by NikitaEdel (Talk | contribs)


mVenus2 with N-terminal spyCatcher

mVenus2 is with mTurqouise2(BBa_K4229064) one of our reporter genes, with which we tested the building and catching of the minimal(BBa_K4229047) and full wiffleball(BBa_K4229049) and SPD5 (BBa_K4229078). It has the spyCatcher fused on the N-terminal of the protein to make it recruitable inside those compartments.

Principle of catching proteins via Spy/Snp-Catcher by T1 and their incorporation into the BMCs, illustrated as an example of incorporating mVenus in the full wiffleball



















Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 982
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 415
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None