Composite

Part:BBa_K4509569

Designed by: MARIE JYOTSNA JAWAHAR, Vibha Murali, Rhea Harry William   Group: iGEM22_REC-CHENNAI   (2022-09-28)
Revision as of 07:59, 12 October 2022 by Rhea3957 (Talk | contribs)


HORSERADISH PEROXIDASE with constitutive promoter J23100

The enzyme horseradish peroxidase, found in the roots of horseradish catalyzes the oxidation of various organic substrates by hydrogen peroxide. It is a metalloenzyme with many isoforms; the most studied type is C. The composite part starts with the BBa_J23100 constitutive promoter, continues with RBS BBa_B0034 and is followed by the Horseradish Peroxidase coding sequence BBa_K1291571. The sequence ends with a terminator and a BamH1 restriction site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 445
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 565
    Illegal AgeI site found at 719
    Illegal AgeI site found at 1022
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None