Part:BBa_K4385014
Lead constitutive surface display
Lead constitutive surface display consists of the constitutive promoter J23100, the lead-inducible regulatory protein PbrR and the anchoring protein Lpp-OmpA.
Usage and Biology
J23100 continuously expresses PbrR for lead ion adsorption, and Lpp-OmpA is used as an anchoring protein to anchor PbrR on the surface of E. coli to construct a whole-cell biosorption device for lead.
Characterization
To verify the sensitivity of Pb2+-inducible promoter, a GFP was connected to report the expression intensity. Pbr-PbrR-Pbc-GFP was constructed to pSB1C3 (in Fig.1)
Different concentrations of Pb2+ were added to the reaction system, and the response of the promoter were reflected by detecting the change of fluorescence intensity. As shown in Fig. 2B, the expression of Pb2+-inducible promoter was optimal under 100 μM Pb2+ after being induced 12 hrs. Moreover, the Pb2+ inducible promoter continues to be effective after 16hours of induction, which shows our system’s stability.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 938
Illegal PstI site found at 880 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1120
Illegal NheI site found at 1143
Illegal SpeI site found at 938
Illegal PstI site found at 880 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1255
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 938
Illegal PstI site found at 880 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 938
Illegal PstI site found at 880 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 671
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