Composite
Part:BBa_K4169030
Designed by: Ruixuan Zhu Group: iGEM22_HZAU-China (2022-10-11)
Revision as of 02:54, 12 October 2022 by Ruixuan Zhu (Talk | contribs)
Cre-reverse loxP system:working when you need to express genes in the other direction
In the presence of theophylline, expression of subsequent Cre recombinase is induced.In addition, the promoter and RBS between the two loxP will be reversed, making it express genes in the other direction.
Usage and Biology
When our engineered bacteria feel theophylline, Cre expresses it, Cre recognizes the reverse repeat sequence at both ends of the loxP site and combines to form a dimer, which then binds to the dimer on another loxP site to form a tetramer. This way Cre mediates the reverse sequence reversal between loxPs.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1292
Illegal NheI site found at 1315 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 454
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 39
Illegal AgeI site found at 140 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |