DNA

Part:BBa_K4438606

Designed by: Neha Adarsh   Group: iGEM22_IISER-Tirupati_India   (2022-10-11)
Revision as of 11:43, 12 October 2022 by Shreyasparkhie17 (Talk | contribs)

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T6_Target_3

T6_Target_3 is a single-stranded DNA sequence having 84 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 3’ end has complementary nucleotides for part T6_Trigger__phi29 (BBa_K4438601) to bind. The middle region has ssDNA antisense T7 promoter sequence and the 5’ end has ssDNA antisense (cDNA) broccoli light-up aptamer sequence.


Usage and Biology

This part along with T6_Trigger_3_phi29 (BBa_K4438605) and Aptamer_T6 (BBa_K4438600) [1] can be used for sensing different concentrations of testosterone. T--IISER-Tirupati India--T6 3.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 76
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 76
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 76
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 76
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Skouridou, V., Jauset-Rubio, M., Ballester, P., Bashammakh, A. S., El-Shahawi, M. S., Alyoubi, A. O., & O’Sullivan, C. K. (2017). Selection and characterization of DNA aptamers against the steroid testosterone. Microchimica Acta, 184(6), 1631-1639.

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