Part:BBa_K4438600:Experience

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Applications of BBa_K4438600

The graph depicts fluorescence readout of T6-D1 and T6-D2 after 30 mins and 1 hour incubation respectively.Dye: DFHBI dye + buffer; T6LA1: DFHBI dye + buffer + Trigger1-T6 Aptamer + Testosterone; T6LA2: DFHBI dye + buffer +Trigger2-T6 Aptamer + Testosterone

T6-Design 1: The fluorescence readout (Fig 2) indicates that the fold change in the fluorescence is not significant. Hence, there is no significant detection of testosterone. T6-Design 2: The fluorescence readout (Fig 2) indicates that the fold change in the fluorescence is significant of 4 folds for 1mM of testosterone. This result shows that T6-D3

After 30 minutes of incubation with 5X sensor buffer and dye, there was a shift in fluorescence intensity for 1mM testosterone compared to lower testosterone concentrations. An maximum fold-change of 4.23 was observed in the sample with 1mM testosterone, while the range of fold-change at lower concentrations was 1.15 to 1.34 folds.

This experiment paved the way for further optimization of testosterone-sensing dual aptamer systems. Our designs proved successful in detecting the hormone, however, further experiments could help us achieve a more sensitive system.

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