DNA

Part:BBa_K4438127

Designed by: Diya Raj N   Group: iGEM22_IISER-Tirupati_India   (2022-10-11)
Revision as of 09:45, 12 October 2022 by Shreyasparkhie17 (Talk | contribs)

FASTmiR-146a-D3_s

FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. This part is the expression cassette for BBa_K4438107, with BBa_J64997 upstream and BBa_K731721 downstream, along with the spacer sequences BBa_K3831027(at 5’ end) and BBa_K4438136(at 3’end). FASTmiR-146a-D3_s, after transcription, binds to miR-146a, the transcription product of No part name specified with partinfo tag.


Notes

The split position of the complementary sequence in the sensor is CTGAAGAACT….GAATTTCAGAGG

References

Huang, K., Doyle, F., Wurz, Z. E., Tenenbaum, S. A., Hammond, R. K., Caplan, J. L., & Meyers, B. C. (2017). FASTmiR: an RNA-based sensor for in vitro quantification and live-cell localization of small RNAs. Nucleic acids research, 45(14), e130-e130

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