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DNA
Part:BBa_K4438112
Designed by: Neha Adarsh Group: iGEM22_IISER-Tirupati_India (2022-10-11)
Revision as of 00:17, 12 October 2022 by Neha adarsh (Talk | contribs)
FASTmiR-30c-D3
FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. FASTmiR-30c-D3, after transcription, binds to miR-30c, the transcription product of BBa_K4438503. The binding of these to RNAs will lead to the revelation of a binding site for DFHBI, a fluorophore, where it gets trapped and shows fluorescence.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 52
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 52
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 52
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 52
- 1000COMPATIBLE WITH RFC[1000]
References
Huang, K., Doyle, F., Wurz, Z. E., Tenenbaum, S. A., Hammond, R. K., Caplan, J. L., & Meyers, B. C. (2017). FASTmiR: an RNA-based sensor for in vitro quantification and live-cell localization of small RNAs. Nucleic acids research, 45(14), e130-e130
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Categories
Parameters
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