Part:BBa_K4201005:Design

Cyto-TDS2

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Unknown
• 12
  INCOMPATIBLE WITH RFC[12]

  Unknown
• 21
  INCOMPATIBLE WITH RFC[21]

  Unknown
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2311
  

Design Notes

Codon optimization for Glycine max (soybean) is a unique design consideration for all of CU Boulder’s parts originating from amino acid sequences, including our cytoTDS2. Codon optimization is the intentional use of specific codons for specific amino acids, dependent on what tRNAs are most abundant in the organism. While codon optimization is a common consideration for synthetic biologists, our sequences are unique for iGEM because they are intended for expression in soybeans.

A diagram of the metabolic pathway for the synthesis of paclitaxel. Taxadiene synthase converts GGPP into Taxadiene, outlined in red.

Source

This taxadiene Synthase sequence originates from Taxus chinensis var. mairei. All of our DNA fragments are obtained via de novo synthesis by iGEM sponsors Twist Bioscience and Integrated DNA technologies.

References

1. Zou, D. et al. Production of a novel lycopene-rich soybean food by fermentation with Bacillus amyloliquefaciens. LWT 153, 112551 (2022).
2. De La Peña, R. & Sattely, E. S. Re-routing plant terpene biosynthesis enables momilactone pathway elucidation. Nat. Chem. Biol. 17, 205–212 (2021).
3. Xiong, X. et al. The Taxus genome provides insights into paclitaxel biosynthesis. Nat. Plants 7, 1026–1036 (2021).