Part:BBa_K4160017

STAT-induced IL-10

This composite part encodes for STAT-induced IL-10 and is composed of the basic parts STAT promotor (BBa_K4160006) and IL-10 (BBa_K4160007).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 209
  Illegal XbaI site found at 233
  Illegal PstI site found at 53
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 209
  Illegal PstI site found at 53
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 209
  Illegal BamHI site found at 227
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 209
  Illegal XbaI site found at 233
  Illegal PstI site found at 53
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 209
  Illegal XbaI site found at 233
  Illegal PstI site found at 53
  
• 1000
  COMPATIBLE WITH RFC[1000]

Usage & biology

TU-Eindhoven 2022 used this composite part as an output of the Generalized Extracellular Molecule Sensor (GEMS) system that is developed by Scheller et al., 2018.¹ Upon activation of the GEMS receptor, STAT-induced IL-10 will be expressed and secreted via the JAK/STAT signaling pathway. In the project of TU-Eindhoven 2022, IL-10 was used to achieve the expression of a therapeutically relevant agent upon activation of the GEMS receptor (BBa_K4160008). STAT-induced IL-10 was expressed in a pLS13 plasmid.

Characterization

Characterization of STAT-induced IL-10 expression by the GEMS system can be found on the BBa_K4160008 page.

References

1. Scheller L, Strittmatter T, Fuchs D, Bojar D, Fussenegger M. Generalized extracellular molecule sensor platform for programming cellular behavior. Nat Chem Biol. Published online 2018. doi:10.1038/s41589-018-0046-z