Part:BBa_K4275005

MtCDH-t

MtCDH-t, fused with a dockerin, is a cellobiose dehydrogenase, one of the five cellulose-related enzymes fixed on type I cohesin, and thus on the scaffold composed of CipA2B9C and OlpB. Its reductive nature enables it to act as an electron donor to the “cellulase booster”, TaLPMO-t, another cellulose-related enzyme on the scaffold which boosts the efficiency of crystalline cellulose degradation. By fusing a type 1 dockerin through a CBM and a 36-bp glycine-rich linker at the C terminal of MtCDH, the free fungal reductase is converted into the cellulosomal mode. Synergizing with the other four cellulose-related enzymes and cellulose binding modules, MtCDH-t is an important contributor to the enhanced efficiency of cellulose degradation. This is a part in a part collection where we enable efficient degradation of cellulose in textile waste.

Figure 1 The 3D structure of the protein predicted by Alphafold2.

Usage and Biology

MtCDH-t, fused with a dockerin, is a cellobiose dehydrogenase that enhances cellulose degradation by coupling the oxidation of cellobiose to the reductive activation of polysaccharide monooxygenases (PMO) that catalyze the insertion of oxygen into C−H bonds adjacent to the glycosidic linkage. MtCDH has a heme domain at the N-terminal and a flavin domain at the C-terminal. The flavin domain is the site of oxidation of cellobiose, and subsequently, electrons are transferred to the heme domain. The reduced heme domain reduced the PMOs. MtCDH-t is fused with a type 1 dockerin through a CBM and a 36-bp glycine-rich linker, thus can bind to type 1 cohesin of the scaffold, immobilizing the enzyme.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2706
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1507
  Illegal AgeI site found at 358
  Illegal AgeI site found at 745
  Illegal AgeI site found at 871
  Illegal AgeI site found at 1420
  
• 1000
  COMPATIBLE WITH RFC[1000]