Regulatory

Part:BBa_K180000:Design

Designed by: Richard Wilson, Stephanie Chambers   Group: iGEM09_Southampton   (2009-10-12)
Revision as of 14:20, 12 October 2009 by Rich (Talk | contribs) (→‎References)

Hybrid promoter (trp & lac regulated -- tac pR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence includes lacIq and Shine-Dalgarno sequence from pMAL-pIII prior to the Ptac promoter to ensure correct functionality of the promoter.


Source

pMAL-pIII (New England Biolabs N8101S)

References

[1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25