Part:BBa_K4165013
UBE2V2
Ubiquitin-conjugating E2 ligase that has a role in the ubiquitination cascade for protein degradation.
Usage and Biology
This gene encodes a homolog of ubiquitin-conjugating enzyme E2 variant 1. These ubiquitin-conjugating enzymes don’t have the conserved cysteine residue critical for the catalytic activity of E2s. Based on the specific E2 used, the E2 enzymes can direct the ubiquitination process to different subsets of ubiquitin lysines. The formation of UBE2N/UBE2V2 complex facilitates the elongation of ubiquitination to forma a ployubiquitin chain. In our case, we will use its interaction with the UBE2N (E2 ligase) to catalyze the formation of polyubiquitin chains and the degradation of our targeted proteins.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
WetLab Results
Transformation of His UBE2V2 in BL-21 using pGS-21a vector
Figure 1. Transformed plate of His UBE2v2 + pGS-21a
Transformation of His UBE2V2 in DH-5 alpha
Figure 2. Transformed plate of His UBE2V2 + pJET
Refrences
1. UBE2V2 ubiquitin conjugating enzyme E2 v2 [homo sapiens (human)] - gene - NCBI. (n.d.). Retrieved September, from https://www.ncbi.nlm.nih.gov/gene/7336
2. David, Y., Ziv, T., Admon, A., & Navon, A. (2010). The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines. Journal of Biological Chemistry, 285(12), 8595-8604.
3. Pharos: Ubiquitin-conjugating enzyme E2 N (Tchem). (2022).
4. Vittal, V., Wenzel, D. M., Brzovic, P. S., & Klevit, R. E. (2013). Biochemical and structural characterization of the ubiquitin-conjugating enzyme UBE2W reveals the formation of a noncovalent homodimer. Cell biochemistry and biophysics, 67(1), 103-110.
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