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Part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein
Usage and Biology
Synthetic peptide acquired by Phage display, Binds specifically to PDZ domain of HTRA1 and HTRA3 Proteins with ΔG of –8.0 kJ.mol-1 and IC50 of 2.8 ± 0.3.
Sequence and Features
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Dry Lab Characterization
Modeling
Got score of 5 out of 6 in our Quality assessment code after modeling by AlphaFold2.0
| cbeta_deviations | clashscore | molprobity | ramachandran_favored | ramachandran_outliers | Qmean_4 | Qmean_6 |
|---|---|---|---|---|---|---|
| 0 | 0 | 1.24 | 80 | 20 | -1.332902 | -0.484265 |
Figure 1.: 3D Model of H1E peptide Modelled by AlphaFold2.0 displayed in Pymol.
References
1.Romero-Molina, S., Ruiz-Blanco, Y. B., Mieres-Perez, J., Harms, M., Münch, J., Ehrmann, M., & Sanchez-Garcia, E. (2022). PPI-Affinity: A Web Tool for the Prediction and Optimization of Protein–Peptide and Protein–Protein Binding Affinity. Journal of Proteome Research.
2.Runyon, S. T., Zhang, Y., Appleton, B. A., Sazinsky, S. L., Wu, P., Pan, B., ... & Sidhu, S. S. (2007). Structural and functional analysis of the PDZ domains of human HtrA1 and HtrA3. Protein Science, 16(11), 2454-2471.
Status: 500 Content-type: text/html
Software error:
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Software error:
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 230, near ""<BR>$group_name, $year, $team_name, $team_digits, $range_name, $range_prefix")" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/extensions/part_box.cgi line 9. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/extensions/part_box.cgi line 9.
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