Coding
Part:BBa_K4488018
Designed by: Jessica Zhang Group: iGEM22_Sydney_Australia (2022-09-30)
Revision as of 22:46, 11 October 2022 by Ctwudonovan (Talk | contribs)
fuBFP
freeuse Blue Fluorescent Protein, a blue fluorescent protein that fluoresces under long-wave UV.
This part is a variant of fuGFP (Part:BBa K3814004) which was originally designed to create a GFP variant not protected by patents and able to facilitate any kind of research. Using PCR with degenerate primers we introduced the Y67H mutation in the fluorophore to create fuBFP. The variant has an excitation (380 nm) and emission peak (450 nm) at a shorter wavelength (figure 1). We have also created a fusion protein of fuBFP connected to a cellulose-binding domain which allows it to be immobilised on cellulose (Part:BBa K4488021).
Usage and Biology
File:Insert file name.png
Figure 1. Absorption and emission spectrum for fuBFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 151
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
| None |