Coding
Part:BBa_K4169000
Designed by: Jie Min Group: iGEM22_HZAU-China (2022-09-29)
Nattokinase gene with pelB
Signal peptide gene of PelB can direct aprN to Escherichia coli periplasmic membrane. The propeptide has been proved to function as an intramolecular chaperone and is essential for guiding the correct folding of the linked protein to its mature form. The nattokinase has potent fibrinolytic activity, which could enhance and prolong fibrinase in the plasma. Based on its food origin and relatively strong fibrinolytic activity, it a possible safe and efficient medicine for use in the prevention of thrombosis diseases.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 202
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 54
Illegal AgeI site found at 685 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
biology |