Coding

Part:BBa_K4165215

Designed by: Engy Khaled   Group: iGEM22_CU_Egypt   (2022-10-07)
Revision as of 08:37, 13 October 2022 by Omnia Alaa11 (Talk | contribs)

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Coh2 linked with tau binding peptide (TD28) & (G4S)3 linker

Fusion protein consists of Tau binding peptide (TD28) for tau targeting and Coh2 protein [BBa_K4165003] that bind to Docs [BBa_K3396000] to form the protac system.

Usage and Biology

Protac system is known to be a promising one for targeting and signaling many diseases and biological application so according to our project aim, we introduced this fusion protein with different linker length and tau binding peptide to be a target for degradation of Tau aggregation in our updated protac version system "Snitch system". Furthermore, that can be validated by wet-lab results by in vitro or in vivo testing.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 469
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 469
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 469
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 469
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None