Part:BBa_K4414007

tdTomato

Usage and Biology

Tandem-dimer tomato (tdTomato) is a stable, robust red fluorescent protein that was modified from previous fluorescence proteins in order to have enhanced fluorescence properties(Syverud et al., 2018) . We obtained this DNA by DNA synthesis and used it in level 1 biological laboratory.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Functional Validation and result

We design plasmids to produce tdTomato, and the pictures below are the fluorescent protein image of the pigment protein in the cell and the cell clump containing the pigment protein under the hydrocoagulant membrane.

Figure 2.The picture on the left is Bright-field cell diagram, the picture in the middle is fluorescence diagram, and the picture on the right is merge diagram.

Reference

1.Syverud, B. C., Gumucio, J. P., Rodriguez, B. L., Wroblewski, O. M., Florida, S. E., Mendias, C. L., & Larkin, L. M. (2018). A Transgenic tdTomato Rat for Cell Migration and Tissue Engineering Applications. Tissue Engineering. Part C, Methods, 24(5), 263–271. https://doi.org/10.1089/ten.TEC.2017.0406