Generator

Part:BBa_K201001

Designed by: francesca ceroni   Group: iGEM09_Bologna   (2009-10-11)
Revision as of 14:11, 21 October 2009 by Francesca ceroni (Talk | contribs)

LacI repressed GFP generator with BBa_K201008

GFP reporter protein was cloned under the control of the BBa_J23100 costitutive promoter from the Berkley library. The promoter is regulated by O2 Lac natural operator .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 734


[edit]
Categories
Parameters
n/aLacI repressed GFP generator with BBa_K201008