Part:BBa_K4169006
HepT: a RNase like toxin
HepT is a toxin that can be effectively inhibited the growth of bacteria.The HepT toxin could function as an RNase with a RX4-6H active motif and cleave mRNA to inhibit cell growth. Complete with HepT (HZAU-China 2021:BBa_K3733010) was used in this study.
Usage and Biology
As an RNase enzyme, HepT can degrade mRNA at the transcriptional level, which ultimately leads to cell death
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 103
Illegal PstI site found at 127 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 103
Illegal PstI site found at 127 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 103
Illegal PstI site found at 127 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 103
Illegal PstI site found at 127 - 1000COMPATIBLE WITH RFC[1000]
References
[1] Yao J, Zhen X, Tang K, et al. Novel polyadenylylation-dependent neutralization mechanism of the HEPN/MNT toxin/antitoxin system[J]. Nucleic acids research, 2020, 48(19): 11054-11067. [2] Yao J, Guo Y, Zeng Z, et al. Identification and characterization of a HEPN‐MNT family type II toxin–antitoxin in S hewanella oneidensis[J]. Microbial biotechnology, 2015, 8(6): 961-973. [3] Jia X, Yao J, Gao Z, et al. Structure–function analyses reveal the molecular architecture and neutralization mechanism of a bacterial HEPN–MNT toxin–antitoxin system[J]. Journal of Biological Chemistry, 2018, 293(18): 6812-6823.
//chassis/prokaryote/ecoli
biology | Escherichia coli |
protein | HepT |