Composite

Part:BBa_K4218003

Designed by: Wuhui Jiang   Group: iGEM22_YiYe-China   (2022-10-07)
Revision as of 16:55, 11 October 2022 by CholeJiang (Talk | contribs)


T7-double Toehold switch-mcherry

Toehold switch is a special RNA hairpin structure, which contains trigger strand, ribosome binding site, translation start codon and a report gene. Our double toehold switch sequence (BBa_BBa_K4218016) is based on an article called Toehold Switches: De-Novo-Designed Regulators of Gene Expression (Green A, et al., 2014) and the mCherry is added to the downstream of the RNA hairpin structure.

Usage and Biology

In our project, the trigger sequence is linked to this part by DNA amplification and was added into the cell free system together with the plasmid contain toehold sequence, the report fluorescent protein will be expressed. The visible color and fluorescent signal can indicate the methylation degree of the TFPI2 gene in CRC patients.

Results

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