Part:BBa_K258011

Oxygen-dependent EGF synthesizing with GFP reporter

The Epidermal Growth Factor(EGF) has a big role in wound healing. However, in our project Wound Dressing,we do not want to synthesize EGF which has cancerogenic features and tumor progression when accumulated too much. Therefore, before unuse of our band-aid,we wanted to stop synthesize these proteins unless the layer on the band-aid is removed to allow oxygen entrance,leading to function as activator of EGF promoter and so KGF synthesize via Quaroum sensing mechanism. That is, if sufficient oxygen entrance is not allowed with removal of the layer on the band-aid, our freeze-dried E.coli will not begin to synthesize EGF. Moreover, it has also a function to start the formation of keratin and to stimulate cell division. It can also increase the amount of nucleic acids.And, it causes the storage of glikozamin and glikozaminoglikan.In structural perspective, it is composed of 53 aminoacids and has three disulfide bond.Although these positive effect of EGF, with the abundance, it can show ,for example, a trigger effect of tumor cells formation. Another side effect is to start the formation of vein.As a result, it may show a trigger for cancer unless unregulated and too much synthesize.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1892
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3339