Coding

Part:BBa_K4367011:Design

Designed by: Anders Kllberg   Group: iGEM22_Chalmers-Gothenburg   (2022-10-11)
Revision as of 19:31, 11 October 2022 by LucasToftas (Talk | contribs) (→‎Design Notes)


FRET Protein (FP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1504
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 14
    Illegal BsaI.rc site found at 1511


Design Notes

This is elaborated in the long description.

Source

It consists of two flourescent proteins, mNeongreen and mRuby3, that are fused together with a cleavable linker. The linker is a typical GS-linker and Tobacco Etch Virus Protease is able to cleave a target sequence within the linker.

References