Coding

Part:BBa_K4461013

Designed by: SHIH-HSUN, YANG   Group: iGEM22_NYCU-Taipei   (2022-10-10)
Revision as of 13:14, 11 October 2022 by YU-TANG-CHANG (Talk | contribs)

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mCherry+tag fusion protein

An improved alternative to mCherry ( BBa_J18932 ) that reduces the fluorescence intensity by fusion with tag( BBa_K1051207 ).

We synthesized a commercial sequence of tag ( BBa_K1051207 ) and EcoRI and HindIII cutting sites on its prefix and suffix. Therefore, we can insert the tag into our construct ( BBa_K4461010 ) by restriction enzyme digestion.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 694
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 694
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 694
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 694
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 694
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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