Part:BBa_K4160000

Igκ secretion signal

Igκ secretion signal is derived from the murine immunoglobulin kappa (IgGκ) light chain (Figure 1). This part is a well-characterized signal peptide for transgene expression¹ and is widely implemented in several eukaryotic expression vectors.² The Igκ secretion signal translocates proteins to the cellular membrane.³

Figure 1 | 3D structure of the IgGκ light chain. Yellow: the Igκ signal. Blue: main chain (AlphaFold: AF-P01661-F1).⁴

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

The Igκ secretion signal is derived from the IgGκ light chain that originates from the Mus musculus (mouse).⁴ This signal is a peptide that influences the targeting pathway of a protein, leading to specific post-translational modifications or translocation of proteins to the cellular membrane.^1,3 Signal peptides are short peptides that consist of approximately 20 to 30 amino acids.¹ It consists of a basic N-domain, a hydrophobic domain, and a slightly polar C-domain.³ Signal peptides are recognized during the translation of a secretory protein. The signal peptide will bind a signal recognition particle, to form a complex that halts the translation. Subsequently, this complex is transported to the endoplasmic reticulum. From there, the translation will resume and the protein will eventually be secreted via the cell's secretory pathway.³ In research, the Igκ secretion signal is often used to express recombinant proteins.² The TU-Eindhoven 2022 used this part to translocate the GEMS receptor (BBa_K4160008) to the membrane of HEK293T cells.

References

1. Fonseca JA, McCaffery JN, Caceres J, et al. Inclusion of the murine IgGκ signal peptide increases the cellular immunogenicity of a simian adenoviral vectored Plasmodium vivax multistage vaccine. Vaccine. 2018;36(20):2799. doi:10.1016/J.VACCINE.2018.03.091
2. Wang X, Liu H, Yuan W, Cheng Y, Han W. Efficient production of CYTL1 protein using mouse IgGκ signal peptide in the CHO cell expression system. Acta Biochim Biophys Sin (Shanghai). 2016;48(4):391. doi:10.1093/ABBS/GMW007
3. Güler-Gane G, Kidd S, Sridharan S, Vaughan TJ, Wilkinson TCI, Tigue NJ. Overcoming the Refractory Expression of Secreted Recombinant Proteins in Mammalian Cells through Modification of the Signal Peptide and Adjacent Amino Acids. PLoS One. 2016;11(5). doi:10.1371/JOURNAL.PONE.0155340
Ig kappa chain V-III region MOPC 63 - Mus musculus (Mouse) | UniProtKB | UniProt. Accessed October 10, 2022. https://www.uniprot.org/uniprotkb/P01661/entry#function