Coding

Part:BBa_K4169015:Design

Designed by: Jie Min   Group: iGEM22_HZAU-China   (2022-09-30)
Revision as of 13:10, 12 October 2022 by Lloheha (Talk | contribs) (→‎References)


produce TMADH


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 388
    Illegal PstI site found at 183
    Illegal PstI site found at 1782
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 388
    Illegal PstI site found at 183
    Illegal PstI site found at 1782
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 388
    Illegal XhoI site found at 1717
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 388
    Illegal PstI site found at 183
    Illegal PstI site found at 1782
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 388
    Illegal PstI site found at 183
    Illegal PstI site found at 1782
    Illegal AgeI site found at 879
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

GenScript synthesised tmd plasmids and performed codon optimization.

We added His tag on the end of tmd sequence.

Source

The gene sequence of tmd is from The European Nucleotide Archive (ENA). Coding: CDX46666.1.

The link is here: https://www.ebi.ac.uk/ena/browser/view/CDX46666

And tmd gene is originally in Mesorhizobium sp. ORS 3359.

References

Jang MH, Basran J, Scrutton NS, Hille R. The reaction of trimethylamine dehydrogenase with trimethylamine. J Biol Chem. 1999 May 7;274(19):13147-54. doi: 10.1074/jbc.274.19.13147. PMID: 10224069.

Loechel C, Basran A, Basran J, Scrutton NS, Hall EA. Using trimethylamine dehydrogenase in an enzyme linked amperometric electrode. Part 1. Wild-type enzyme redox mediation. Analyst. 2003 Feb;128(2):166-72. doi: 10.1039/b211895e. PMID: 12625558.