Coding

Part:BBa_K4169016:Design

Designed by: Jie Min   Group: iGEM22_HZAU-China   (2022-09-30)
Revision as of 02:19, 11 October 2022 by Lloheha (Talk | contribs) (→‎Source)


produce DMADH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 897
    Illegal AgeI site found at 1291
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

GenScript synthesised tmd plasmids and performed codon optimization.

We added His tag on the end of tmd sequence.

Source

The gene sequence of dmd is from The European Nucleotide Archive (ENA). Sequence: X89575.1

The link is here: https://www.ebi.ac.uk/ena/browser/view/X89575

And dmd gene is originally in Hyphomicrobium sp.

References

Yang CC, Packman LC, Scrutton NS. The primary structure of Hyphomicrobium X dimethylamine dehydrogenase. Relationship to trimethylamine dehydrogenase and implications for substrate recognition. Eur J Biochem. 1995 Aug 15;232(1):264-71. doi: 10.1111/j.1432-1033.1995.tb20808.x. PMID: 7556160.

Loechel C, Basran A, Basran J, Scrutton NS, Hall EA. Using trimethylamine dehydrogenase in an enzyme linked amperometric electrode. Part 1. Wild-type enzyme redox mediation. Analyst. 2003 Feb;128(2):166-72. doi: 10.1039/b211895e. PMID: 12625558.