Coding

Part:BBa_K4169016:Design

Designed by: Jie Min   Group: iGEM22_HZAU-China   (2022-09-30)
Revision as of 01:41, 11 October 2022 by Lloheha (Talk | contribs) (→‎References)


produce DMADH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 897
    Illegal AgeI site found at 1291
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

GenScript synthesised tmd plasmids and performed codon optimization.

We added His tag on the end of tmd sequence.

Source

It comes from https://www.ebi.ac.uk/ena/browser/view/X89575 Coding: CDX46666.1.


References

Yang CC, Packman LC, Scrutton NS. The primary structure of Hyphomicrobium X dimethylamine dehydrogenase. Relationship to trimethylamine dehydrogenase and implications for substrate recognition. Eur J Biochem. 1995 Aug 15;232(1):264-71. doi: 10.1111/j.1432-1033.1995.tb20808.x. PMID: 7556160.

Loechel C, Basran A, Basran J, Scrutton NS, Hall EA. Using trimethylamine dehydrogenase in an enzyme linked amperometric electrode. Part 1. Wild-type enzyme redox mediation. Analyst. 2003 Feb;128(2):166-72. doi: 10.1039/b211895e. PMID: 12625558.