Coding

Part:BBa_K4182008:Design

Designed by: Dan Tan   Group: iGEM22_XJTU-China   (2022-10-10)
Revision as of 00:44, 11 October 2022 by Dan012 (Talk | contribs) (→‎Design Notes)


AA cluster


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1877
    Illegal EcoRI site found at 4795
    Illegal PstI site found at 126
    Illegal PstI site found at 306
    Illegal PstI site found at 1413
    Illegal PstI site found at 2744
    Illegal PstI site found at 4552
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1877
    Illegal EcoRI site found at 4795
    Illegal PstI site found at 126
    Illegal PstI site found at 306
    Illegal PstI site found at 1413
    Illegal PstI site found at 2744
    Illegal PstI site found at 4552
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1877
    Illegal EcoRI site found at 4795
    Illegal BamHI site found at 184
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1877
    Illegal EcoRI site found at 4795
    Illegal PstI site found at 126
    Illegal PstI site found at 306
    Illegal PstI site found at 1413
    Illegal PstI site found at 2744
    Illegal PstI site found at 4552
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1877
    Illegal EcoRI site found at 4795
    Illegal PstI site found at 126
    Illegal PstI site found at 306
    Illegal PstI site found at 1413
    Illegal PstI site found at 2744
    Illegal PstI site found at 4552
    Illegal NgoMIV site found at 2015
    Illegal NgoMIV site found at 3345
    Illegal AgeI site found at 2170
    Illegal AgeI site found at 3970
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This gene cluster has been optimized for E. coli

Source

LacI:E.coli FPPS:Rhodobacter azotoformans fpps gene for farnesyl diphosphate synthase, partial cds(GenBank: AB053174.1)

References