Coding

Part:BBa_K209486:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-01)
Revision as of 21:29, 21 October 2009 by Brhau (Talk | contribs) (→‎Source)

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AarI B-D part, DOR-Actinin1ABD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 31
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 31
    Illegal NotI site found at 1010
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 641
    Illegal BglII site found at 1286
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 31
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 31
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cloned into pCR2.1.

Source

Parent plasmid obtained from von Zastrow lab, UCSF.

References