Part:BBa_K4165145

TD28 Peptide

A synthetic peptide that is used for targeting misfolded tau protein (BBa_K4165009) as it binds to PHF6 of tau fibrils.

Usage and Biology

TD peptide (TTSLQMRLYYP) derived from D-enantiomeric peptide acquired by mirror phage display. Its half maximal concentration for tau fibril formation inhibition is 7.9 μM, it can bind to tau at PHF seed VQIVYK and penetrate cell membrane and nuclei.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 10
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 10
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 10
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 10
  
• 1000
  COMPATIBLE WITH RFC[1000]

Dry Lab

Modeling

modeled by AlphaFold2, Apptest, ITASSER, RosettaFold and TrRosetta, best model obtained from AlphaFold2

                              Figure 1.: Predicted 3D structure of Synthetic peptide TD28.

Table 1: Quality assessment parameters of TD28 model.

References

1. Dammers, C., Yolcu, D., Kukuk, L., Willbold, D., Pickhardt, M., Mandelkow, E., ... & Funke, S. A. (2016). Selection and Characterization of Tau Binding ᴅ-Enantiomeric Peptides with Potential for Therapy of Alzheimer Disease. PLoS One, 11(12), e0167432.