Part:BBa_K4195069

pirA_i

This sequence is a conserved region of toxin gene pirA(1). It’s used as the detection target of RENDR system.

Biology

Ribozyme ENabled Detection of RNA (RENDR)
RENDR is a high-performing, plug-and-play RNA-sensing platform(1). RENDR utilizes a split variant of the Tetrahymena thermophila ribozyme by synthetically splitting it into two non-functional fragments (Fig. 1). Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When bound with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form.

Fig. 1 Schematic illustration of RENDR.

Usage and Design

This part is used as the target of the RENDR detection system. For toxin pirA, we designed BBa_K4195141, BBa_K4195142, BBa_K4195145, BBa_K4195146, BBa_K4195156, BBa_K4195157, BBa_K4195160, BBa_K4195161, BBa_K4195168, BBa_K4195169, BBa_K4195172, BBa_K4195173. Other related parts are as following: BBa_K4195151, BBa_K4195183, BBa_K4195184, BBa_K4195185, BBa_K4195186.

Reference

1. J. M. S. Lazarte et al., Passive Immunization with Recombinant Antibody VLRB-PirA(vp)/PirB(vp)-Enriched Feeds against Vibrio parahaemolyticus Infection in Litopenaeus vannamei Shrimp. Vaccines (Basel) 9, (2021). Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]