Device

Part:BBa_K4472999:Design

Designed by: Stine Behrmann   Group: iGEM22_Uni-Hamburg   (2022-10-10)
Revision as of 16:39, 11 October 2022 by StiMaBeh (Talk | contribs) (→‎Design Notes)


Ribozyme half 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 10
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoters of split ribozyme half 1 and split ribozyme half 2 need to be different in order to get them synthesized. If they are the same there is too much repetition for the synthesis companies.

Source

paper

References