Composite

Part:BBa_K4297077

Designed by: Yang Gu   Group: iGEM22_NJXDF-CHN   (2022-10-10)
Revision as of 06:55, 11 October 2022 by GYang1213 (Talk | contribs)


PL-CBE

The composite part contains Lambda cI , cytidine deaminase, Uracil DNA glycosylase inhibitor and LVA degradation tag. CDA catalyzes the deamination of cytosine in the top (non-complementary) strand located within 15-20 bases upstream of PAM, resulting in a C-T mutation.

Fig. The plasmid construction process

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2097
    Illegal NheI site found at 6019
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5432
    Illegal BamHI site found at 4376
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5111
    Illegal AgeI site found at 5757
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 988
    Illegal BsaI site found at 5114
    Illegal BsaI site found at 5760
    Illegal BsaI.rc site found at 5108
    Illegal BsaI.rc site found at 5754
    Illegal BsaI.rc site found at 6028


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Parameters
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