Composite

Part:BBa_K4297053

Designed by: Yang Gu   Group: iGEM22_NJXDF-CHN   (2022-10-10)
Revision as of 06:45, 11 October 2022 by GYang1213 (Talk | contribs)


PYLXP'-ARO10

plasmid pYLXP' containing the expressiom cassette of the phenylpyruvate decarboxylase ARO10

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1293
    Illegal NotI site found at 1448
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5677
    Illegal BglII site found at 5884
    Illegal BamHI site found at 249
    Illegal XhoI site found at 4316
    Illegal XhoI site found at 4349
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4945
    Illegal AgeI site found at 950
    Illegal AgeI site found at 4585
    Illegal AgeI site found at 5535
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1284
    Illegal BsaI site found at 4588
    Illegal BsaI.rc site found at 255
    Illegal BsaI.rc site found at 4582
    Illegal BsaI.rc site found at 5082
    Illegal BsaI.rc site found at 5817
    Illegal BsaI.rc site found at 6407


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Parameters
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