Coding

Part:BBa_K4461013:Experience

Designed by: SHIH-HSUN, YANG   Group: iGEM22_NYCU-Taipei   (2022-10-10)
Revision as of 12:46, 11 October 2022 by YU-TANG-CHANG (Talk | contribs) (→‎Applications of BBa_K4461013)

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Applications of BBa_K4461013

If the plasmid contained glpABC-mCherry with tag, there will be an EcoRI site that we used to insert tag by enzyme digestion and ligation, or it is a plasmid contained mut-glpABC-mCherry, which doesn't have an EcoRI site on it. Therefore, we digested them with EcoRI and BamHI, and the one being digested into 2 bands is correct.

Fig1. After EcoRI/BamHI enzyme digestion, we found the correct plasmid (glpABC-mCherry-tag1).

The fusion protein are tested under the glpABC promoter and compared with that without tag.

Fig.2 The result that we measured the FP intensity and OD600 for the mCherry with and without tag(BBa_K4461505 and BBa_K4461504).

User Reviews

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