Coding

Part:BBa_K4343113

Designed by: Xiaolu Sun   Group: iGEM22_NNU-China   (2022-10-09)
Revision as of 14:15, 10 October 2022 by TripleDye (Talk | contribs) (→‎Description)


Sc4Des

Sc4Des encodes Δ-4 desaturase from Schizochytrium sp. which functions in the Δ-9 elongase/desaturase pathway and could convert C22:5 to C22:6.

Description

The Sc4Des gene encodes a ∆-4 desaturase of 427 amino acids from Schizochytrium sp. that catalyzes the conversion of Docosapentaenoic acid (DPA; C22:5n-3) to Docosahexaenoic acid (DHA; C22:6n- 3).（Fig. The Gel Electrophoretic Image of Sc4Des gene）

sc4des.png

Literature

Some studies have shown that Δ-4 desaturase from Schizochytrium sp.is used to produce Docosahexaenoic acid (DHA).

Reference

Ren, L. J., Zhuang, X. Y., Chen, S. L., Ji, X. J., & Huang, H. (2015). Introduction of ω-3 desaturase obviously changed the fatty acid profile and sterol content of Schizochytrium sp. Journal of agricultural and food chemistry, 63(44), 9770-9776.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 356
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 664
    Illegal BsaI site found at 813
    Illegal BsaI site found at 846
    Illegal BsaI.rc site found at 1301


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Categories
Parameters
None