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Part:BBa_J70503:Design

Designed by: Tom Knight   Group: Knight Lab   (2009-10-03)
Revision as of 01:29, 4 October 2009 by Tk (Talk | contribs) (→‎Design Notes)

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Mesoplasma florum origin of replication


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 928
    Illegal EcoRI site found at 1609
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 928
    Illegal EcoRI site found at 1609
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 928
    Illegal EcoRI site found at 1609
    Illegal BglII site found at 466
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 928
    Illegal EcoRI site found at 1609
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 928
    Illegal EcoRI site found at 1609
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Needs mutation of EcoRI sites and GATC sites.

Source

PCR from genomic DNA

References