Composite

Part:BBa_K209435:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-02)
Revision as of 21:46, 5 October 2009 by Katja (Talk | contribs) (→‎Design Notes)

SSFYFP-hM2D-LPD(775-1250)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
    Illegal PstI site found at 5273
    Illegal PstI site found at 5648
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 239
    Illegal NheI site found at 505
    Illegal NheI site found at 3799
    Illegal PstI site found at 2984
    Illegal PstI site found at 5273
    Illegal PstI site found at 5648
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2766
    Illegal BamHI site found at 706
    Illegal BamHI site found at 3593
    Illegal BamHI site found at 4303
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
    Illegal PstI site found at 5273
    Illegal PstI site found at 5648
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
    Illegal PstI site found at 5273
    Illegal PstI site found at 5648
    Illegal AgeI site found at 5154
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2722
    Illegal BsaI site found at 5618
    Illegal BsaI.rc site found at 5565
    Illegal BsaI.rc site found at 5632
    Illegal BsaI.rc site found at 6305
    Illegal SapI.rc site found at 116


Design Notes

Cloned into pDONR221.

Source

References