Composite

Part:BBa_K209420:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-02)
Revision as of 21:35, 5 October 2009 by Katja (Talk | contribs) (→‎Design Notes)

SSFYFP-Rs1.3-ActA(255-392)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 239
    Illegal NheI site found at 505
    Illegal PstI site found at 2984
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2766
    Illegal BamHI site found at 706
    Illegal BamHI site found at 4590
    Illegal BamHI site found at 4804
    Illegal XhoI site found at 4117
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2722
    Illegal SapI.rc site found at 116


Design Notes

Cloned into pDONR221.

Source

References