Coding

Part:BBa_K4461013:Experience

Designed by: SHIH-HSUN, YANG   Group: iGEM22_NYCU-Taipei   (2022-10-10)
Revision as of 14:32, 10 October 2022 by YU-TANG-CHANG (Talk | contribs) (→‎Applications of BBa_K4461013)


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Applications of BBa_K4461013

If the plasmid contained glpABC-mCherry with tag, there will be an EcoRI site that we used to insert tag by enzyme digestion and ligation, or it is a plasmid contained mut-glpABC-mCherry, which doesn't have an EcoRI site on it. Therefore, we digested them with EcoRI and BamHI, and the one being digested into 2 bands is correct.

Fig1. After EcoRI/BamHI enzyme digestion, we found the correct plasmid (glpABC-mCherry-tag1).

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UNIQbad6dd05b691baf1-partinfo-00000000-QINU UNIQbad6dd05b691baf1-partinfo-00000001-QINU