Generator

Part:BBa_K215211

Designed by: Alex Leone   Group: iGEM09_Washington   (2009-10-01)
Revision as of 01:36, 22 October 2009 by Acleone (Talk | contribs)

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IPTG Inducible 5tmr OmpA Surface Display of WT Streptavidin

This part should display WT streptavidin + 6His, BBa_J36841, on the surface of the cell via the OmpA display system when induced with IPTG.

Note: in order to express this construct as a fusion protein, there is no T after this sequence and before the SpeI site (sequencing will show inconsistent).

This part doesn't show measurable activity. See [http://2009.igem.org/Team:Washington/Project/CDS#Custom_Display_Vector_with_wild-type_monomeric_streptavidin_inserted 2009 UW iGEM page] for more information.

New generic 58.png
Cytometry showing no difference in bound biotin-conjugated fluorophore for 1mM IPTG induced vs uninduced cells at different fluorophore concentrations. Conclusion: Wild-type monomeric streptavidin shows no measureable activity when expressed as a fusion protein with 5 trans-membrane OmpA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 616
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 658
    Illegal AgeI site found at 709
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aIPTG Inducible 5tmr OmpA Surface Display of WT Streptavidin