Composite

Part:BBa_K215107

Designed by: Josef Dunbar   Group: iGEM09_Washington   (2009-09-30)
Revision as of 04:36, 1 October 2009 by Siegeljb (Talk | contribs)

Extracellular Secretion System

This is the 3 genes: prtD, prtE, prtF, that are from a type I Erwinia chrysanthemi secretion system. The operon in under expression by a strong constitutive promoter, BBa_J23100, and has the translational terminator BBa_B0014. In PSB3T5

Used to secrete proteins containing prtB C-terminus tag. The prtB C-terminus tag is built into the protein generator [BBa_K215002]. Any protein of interest can be inserted into the protein generator and then secreted when used in conjunction with this secretion system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1487
    Illegal BsaI.rc site found at 1133
    Illegal BsaI.rc site found at 3737


[edit]
Categories
Parameters
None