Coding

Part:BBa_K4229013

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-28)
Revision as of 05:34, 10 October 2022 by NikitaEdel (Talk | contribs)


TnaA with N-terminal SnoopCatcher


TnaA codes for a tryptophanase. This tryptophanase turns L-tryptophan into either Indol. This biobrick consists of a version of TnaA, which is fused with the snoopcatcher(BBa_K4229009) on the N-terminal of the protein. With that, we made it able to be recruted into the wiffelball we used in our project.

Grafik indigo weier bg.png


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 977
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 977
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 977
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 977
    Illegal AgeI site found at 142
    Illegal AgeI site found at 228
    Illegal AgeI site found at 1600
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None