Generator

Part:BBa_K4325015

Designed by: Laiyi Teng   Group: iGEM22_SZPT-CHINA   (2022-09-30)
Revision as of 03:34, 10 October 2022 by Taaa (Talk | contribs)


J23102-RBS003422-gshF-T0

Description

The composite part is a generator consisting of a J23102 promoter and gshF.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 223
    Illegal EcoRI site found at 805
    Illegal EcoRI site found at 1240
    Illegal EcoRI site found at 1738
    Illegal PstI site found at 64
    Illegal PstI site found at 94
    Illegal PstI site found at 451
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 223
    Illegal EcoRI site found at 805
    Illegal EcoRI site found at 1240
    Illegal EcoRI site found at 1738
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 64
    Illegal PstI site found at 94
    Illegal PstI site found at 451
    Illegal NotI site found at 1883
    Illegal NotI site found at 2083
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 223
    Illegal EcoRI site found at 805
    Illegal EcoRI site found at 1240
    Illegal EcoRI site found at 1738
    Illegal XhoI site found at 2064
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 223
    Illegal EcoRI site found at 805
    Illegal EcoRI site found at 1240
    Illegal EcoRI site found at 1738
    Illegal PstI site found at 64
    Illegal PstI site found at 94
    Illegal PstI site found at 451
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 223
    Illegal EcoRI site found at 805
    Illegal EcoRI site found at 1240
    Illegal EcoRI site found at 1738
    Illegal PstI site found at 64
    Illegal PstI site found at 94
    Illegal PstI site found at 451
    Illegal NgoMIV site found at 1665
    Illegal NgoMIV site found at 2296
  • 1000
    COMPATIBLE WITH RFC[1000]


2022 SZPT-China

1.Characterization in E. coli TOP10

As shown in Figure 1, composite part J23102-RBS0034-gshF-T0 and the expression of GshF were verified successfully by PCR amplification and SDS-PAGE respectively. As well as GSH production was detected.

Figure 2:

(a) Verification of gshF in E. coli

(b) Verification of SDS-PAGE electrophoresis in E. coli.

(c) Comparison of GSH production between wild type and engineered bacteria of E. coli .
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