Composite

Part:BBa_K4388006:Experience

Designed by: Tomas Lopes   Group: iGEM22_KCL_UK   (2022-10-09)
Revision as of 19:35, 11 October 2022 by KalyanGhadiyaram (Talk | contribs) (→‎Applications of BBa_K4388006)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4388006

KCL iGEM 2022

Through JUMP type IIS Level 1, this transcriptional unit (BBa_K4388006) was assembled in a one-pot digestion reaction with BsaI and ligation into pJUMP29-1B. Successful ligation and transformation were selected for by identifying non-fluorescent colonies on kanamycin agar plates (Fig. 1).

TU2 resized.png

Figure 1. Construction of Transcriptional Unit (TU) BBa_K4388006 into Level 1 pJUMP29-1B vector. Level 0 parts BBa_K4388011, BBa_K4388002, and BBa_K4388012 were assembled into the transcriptional unit BBa_K4388006 in pJUMP29-1B via a one-pot BsaI digestion reaction. White (sfGPF-excised) colonies indicate successful ligation of the TU into plasmid, and successful transformation of plasmid conferring kanamycin resistance. Final level 1 plasmid constructs were designed using SnapGene.

User Reviews

UNIQ966a4f8ae8b24518-partinfo-00000000-QINU UNIQ966a4f8ae8b24518-partinfo-00000001-QINU