Tag

Part:BBa_K215001:Design

Designed by: Jeff Nivala   Group: iGEM09_Washington   (2009-09-30)
Revision as of 03:26, 1 October 2009 by Siegeljb (Talk | contribs) (→‎Source)

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Secretion Signal and Streptavidin Binding Tags


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 115
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NheI was chosen as the protein insertion site because it is compatible with digested XbaI and SpeI restriction sites. The TEV sites and HIS tags flanking the NheI site are present to separate the inserted protein from the added tags. GSA linkers were used to separate the various features of the part.


Source

Nanotag (bases 1-48): From Lamla and Erdmann (http://www.ncbi.nlm.nih.gov/pubmed/14680960). Secretion tag (181 C-terminal amino acids of prtB): Gene Bank ID Erwinia chrysanthemi M60395.1

References