Coding

Part:BBa_K4497011

Designed by: Till Gundlach   Group: iGEM22_Munich   (2022-10-09)
Revision as of 14:07, 13 October 2022 by Till Gundlach (Talk | contribs)

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TEV MESA Part

This part encodes the intracellular protease function of the MESA system. We used it in combination with mCherry and GFP nanobodies (see Part:Bba_K4497017).

Design & Cloning

Design

This part is made of the following components:

  • CD 28 transmembrane domain (Part:BBa_K4497004): transmembrane anchor for the MESA system
  • Tobacco Etch Virus (TEV) Protease (Part:BBa_K4497005): Protease cleaving proteins the TEV recognition site

Cloning

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 448
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 448
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 448
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 448
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None