Coding

Part:BBa_K4461100:Design

Designed by: CHIH-HO, WANG   Group: iGEM22_NYCU-Taipei   (2022-10-09)
Revision as of 14:34, 10 October 2022 by YU-TANG-CHANG (Talk | contribs) (→‎References)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


ftsQp1+ftsQp2 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We cloned a larger fragment that include the promoter, then use another pair of primers to specify clone of the promoter. Therefore we can avoid the length of the unintended bands being too similar to distinct easily. The sequence we cloned has to include all transcriptional factors for the promoter. We use BBa_B0034 as the RBS of the promoter, so we didn't clone the RBS from the genomic DNA.



Source

The ftsQp1 and ftsQp2 promoters are cloned from E.coli K-12 MG1655 genomic DNA.


References

[1]Flärdh K, Garrido T, Vicente M. Contribution of individual promoters in the ddlB-ftsZ region to the transcription of the essential cell-division gene ftsZ in Escherichia coli. Mol Microbiol. 1997 Jun;24(5):927-36. PMID: 9220001.

[2]Garrido T, Sánchez M, Palacios P, Aldea M, Vicente M. Transcription of ftsZ oscillates during the cell cycle of Escherichia coli. EMBO J. 1993 Oct;12(10):3957-65. PMID: 8404863; PMCID: PMC413678.